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| Registros recuperados : 3 | |
| 1. |  | VIBRANS, A. C.; UHLMANN, A.; SEVEGNANI, L.; MARCOLIN, M.; NAKAJIMA, N.; GRIPPA, C. R.; BROGNI, E.; GODOY, M. B. Ordenação dos dados de estrutura da Floresta Ombrófila Mista partindo de informações do inventário florístico-florestal de Santa Catarina: resultados de estudo-piloto. Ciência Florestal, Santa Maria, v. 18, n.3/4, p. 511-523, set./dez. 2008.| Biblioteca(s): Embrapa Florestas. |
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| 2. | | MOREIRA, F. M. de S.; LIMA, A. S.; ACIOLI, A. N. S.; MOINO JUNIOR, A.; TRGUE, A. P.; BARBERI, A.; MARTINS, A. L. U.; NOGUEIRA, C. L. B.; GRIPPA, C. R.; PEREZ, D. V.; SILVA FILHO, D. F. da; ALBUQUERQUE, D. C. de; JESUS, E. da C.; ANDRADE, E. P.; FIDALGO, E. C. C.; ARAUJO, F. de O.; VILLANI, F. T.; MACHADO, F. M.; RIBEIRO, F. V.; RIBEIRO, G.; SILVA, G. A. e; SCHIFFLER, G.; PEREIRA, H.; NODA, H.; SANTOS, H. G. dos; AMARAL, I. L. do; DELABIE, J.; SIQUEIRA, J. O.; MORAIS, J. W.; PEREIRA, J. P. R.; VIDAL, J. de O.; LOUZADA, J.; CARES, J.; UGUEN, K.; SILVA, K. de; FLORENTINO, L. A.; ABREU, L. M. de; PFENNING, L. H.; OLIVEIRA, M. S.; GOMIDE, M. L.; MESQUITA, M. da G. B. de; BREFIN, M. de L. M. S.; RODRIGUES, M. I. D.; COELHO, M. R.; SILVA, M. A.; SANTOS, M. S.; SILVA, N. M. da; DIAS, N.; NÓBREGA, R. S. A.; CONSTANTINO, R.; CAVALCANTI, R. S.; OLIVEIRA, R. F. M. de; LEAL, P. L.; FERREIRA, P. A.; SILVA, P. H.; ZANETTI, R.; TAPIA-CORAL, S. C.; STUMER, S. L.; ALFAIA, S. S.; ANDALÓ, V.; OLIVEIRA, V. dos S. Biodiversidade do solo em sistemas de uso da terra na Amazônia ocidental. In: PERES, C. A.; BARLOW, J.; GARDNER, T. A.; VIEIRA, I. C. G. (Org.). Conservação da biodiversidade em paisagens antropizadas do Brasil. Curitiba: UFPR, 2013. cap. 14, p. 293-326.| Biblioteca(s): Embrapa Solos. |
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| 3. | | MOREIRA, F. M. de S.; LIMA, A. S.; ACIOLI, A. N. S.; MOINO JUNIOR, A.; TRGUE, A. P.; BARBERI, A.; MARTINS, A. L. U.; NOGUEIRA, C. L. B.; GRIPPA, C. R.; PEREZ, D. V.; SILVA FILHO, D. F. da; ALBUQUERQUE, D. C. de; JESUS, E. da C.; ANDRADE, E. P.; FIDALGO, E. C. C.; ARAUJO, F. de O.; VILLANI, F. T.; MACHADO, F. M.; RIBEIRO, F. V.; RIBEIRO, G.; SILVA, G. A. e; SCHIFFLER, G.; PEREIRA, H.; NODA, H.; SANTOS, H. G. dos; AMARAL, I. L. do; DELABIE, J.; SIQUEIRA, J. O.; MORAIS, J. W.; PEREIRA, J. P. R.; VIDAL, J. de O.; LOUZADA, J.; CARES, J.; UGUEN, K.; SILVA, K. de; FLORENTINO, L. A.; ABREU, L. M. de; PFENNING, L. H.; OLIVEIRA, M. S.; GOMIDE, M. L.; MESQUITA, M. da G. B. de; BREFIN, M. de L. M. S.; RODRIGUES, M. I. D.; COELHO, M. R.; SILVA, M. A.; SANTOS, M. S.; SILVA, N. M. da; DIAS, N.; NÓBREGA, R. S. A.; CONSTANTINO, R.; CAVALCANTI, R. S.; OLIVEIRA, R. F. M. de; LEAL, P. L.; FERREIRA, P. A.; SILVA, P. H.; ZANETTI, R.; TAPIA-CORAL, S. C.; STUMER, S. L.; ALFAIA, S. S.; ANDALÓ, V.; OLIVEIRA, V. dos S. Biodiversidade do solo em sistemas de uso da terra na Amazônia ocidental. In: PERES, C. A.; BARLOW, J.; GARDNER, T. A.; VIEIRA, I. C. G. (Org.). Conservação da biodiversidade em paisagens antropizadas do Brasil. Curitiba: UFPR, 2013. 38p.| Biblioteca(s): Embrapa Agrobiologia. |
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| Registros recuperados : 3 | |
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Registro Completo
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Biblioteca(s): |
Embrapa Recursos Genéticos e Biotecnologia; Embrapa Semiárido. |
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Data corrente: |
18/09/2020 |
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Data da última atualização: |
17/11/2020 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 1 |
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Autoria: |
MELO, B. P. de; LOURENCO-TESSUTTI, I. T.; MORGANTE, C. V.; SANTOS, N. C.; PINHEIRO, L. B.; LINS, C. B. de J.; SILVA, M. C. M.; MACEDO, L. L. P.; FONTES, E. P. B.; GROSSI-DE-SA, M. F. |
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Afiliação: |
BRUNO PAES DE MELO, UFV; ISABELA TRISTAN LOURENCO TESSUTTI, Cenargen; CAROLINA VIANNA MORGANTE, CPATSA; NAIARA CORDEIRO SANTOS; LUANNA BEZERRA PINHEIRO, UCB; CAMILA BARROZO DE JESUS LINS; MARIA CRISTINA MATTAR DA SILVA, Cenargen; LEONARDO LIMA PEPINO DE MACEDO, Cenargen; ELIZABETH PACHECO BATISTA FONTES, UFV; MARIA FATIMA GROSSI DE SA, Cenargen. |
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Título: |
Soybean embryonic axis transformation: combining biolistic and Agrobacterium-Mediated Protocols to overcome typical complications of in vitro plant regeneration. |
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Ano de publicação: |
2020 |
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Fonte/Imprenta: |
Frontiers in Plant Science, v. 11, article 1228, 2020. |
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DOI: |
https://doi.org/10.3389/fpls.2020.01228 |
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Idioma: |
Inglês |
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Conteúdo: |
The first successful attempt to generate genetically modified plants expressing a transgene was preformed via T-DNA-based gene transfer employing Agrobacterium tumefaciens-mediated genetic transformation. Limitations over infectivity and in vitro tissue culture led to the development of other DNA delivery systems, such as the biolistic method. Herein, we developed a new one-step protocol for transgenic soybean recovery by combining the two different transformation methods. This protocol comprises the following steps: agrobacterial preparation, seed sterilization, soybean embryo excision, shoot-cell injury by tungsten-microparticle bombardment, A. tumefaciens-mediated transformation, embryo co-cultivation in vitro, and selection of transgenic plants. This protocol can be completed in approximately 30?40 weeks. The average efficiency of producing transgenic soybean germlines using this protocol was 9.84%, similar to other previously described protocols. However, we introduced a more cost-effective, more straightforward and shorter methodology for transgenic plant recovery, which allows co-cultivation and plant regeneration in a single step, decreasing the chances of contamination and making the manipulation easier. Finally, as a hallmark, our protocol does not generate plant chimeras, in contrast to traditional plant regeneration protocols applied in other Agrobacterium-mediated transformation methods. Therefore, this new approach of plant transformation is applicable for studies of gene function and the production of transgenic cultivars carrying different traits for precision-breeding programs. MenosThe first successful attempt to generate genetically modified plants expressing a transgene was preformed via T-DNA-based gene transfer employing Agrobacterium tumefaciens-mediated genetic transformation. Limitations over infectivity and in vitro tissue culture led to the development of other DNA delivery systems, such as the biolistic method. Herein, we developed a new one-step protocol for transgenic soybean recovery by combining the two different transformation methods. This protocol comprises the following steps: agrobacterial preparation, seed sterilization, soybean embryo excision, shoot-cell injury by tungsten-microparticle bombardment, A. tumefaciens-mediated transformation, embryo co-cultivation in vitro, and selection of transgenic plants. This protocol can be completed in approximately 30?40 weeks. The average efficiency of producing transgenic soybean germlines using this protocol was 9.84%, similar to other previously described protocols. However, we introduced a more cost-effective, more straightforward and shorter methodology for transgenic plant recovery, which allows co-cultivation and plant regeneration in a single step, decreasing the chances of contamination and making the manipulation easier. Finally, as a hallmark, our protocol does not generate plant chimeras, in contrast to traditional plant regeneration protocols applied in other Agrobacterium-mediated transformation methods. Therefore, this new approach of plant transformation is applicable for stud... Mostrar Tudo |
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Palavras-Chave: |
Agrobacterium-mediated transformation; Embryonic axis; High-efficiency plant transformation; Particle bombardment; Planta geneticamente modificada; Recuperação trangênica de soja. |
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Thesagro: |
Cultura de Tecido; Glycine Max; Soja. |
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Thesaurus NAL: |
Agrobacterium; Embryonic structures; Genetic transformation; Plant genetics. |
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Categoria do assunto: |
--
G Melhoramento Genético |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/216085/1/fpls-11-01228.pdf
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Marc: |
LEADER 02941naa a2200397 a 4500
001 2125013
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008 2020 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.3389/fpls.2020.01228$2DOI
100 1 $aMELO, B. P. de
245 $aSoybean embryonic axis transformation$bcombining biolistic and Agrobacterium-Mediated Protocols to overcome typical complications of in vitro plant regeneration.$h[electronic resource]
260 $c2020
520 $aThe first successful attempt to generate genetically modified plants expressing a transgene was preformed via T-DNA-based gene transfer employing Agrobacterium tumefaciens-mediated genetic transformation. Limitations over infectivity and in vitro tissue culture led to the development of other DNA delivery systems, such as the biolistic method. Herein, we developed a new one-step protocol for transgenic soybean recovery by combining the two different transformation methods. This protocol comprises the following steps: agrobacterial preparation, seed sterilization, soybean embryo excision, shoot-cell injury by tungsten-microparticle bombardment, A. tumefaciens-mediated transformation, embryo co-cultivation in vitro, and selection of transgenic plants. This protocol can be completed in approximately 30?40 weeks. The average efficiency of producing transgenic soybean germlines using this protocol was 9.84%, similar to other previously described protocols. However, we introduced a more cost-effective, more straightforward and shorter methodology for transgenic plant recovery, which allows co-cultivation and plant regeneration in a single step, decreasing the chances of contamination and making the manipulation easier. Finally, as a hallmark, our protocol does not generate plant chimeras, in contrast to traditional plant regeneration protocols applied in other Agrobacterium-mediated transformation methods. Therefore, this new approach of plant transformation is applicable for studies of gene function and the production of transgenic cultivars carrying different traits for precision-breeding programs.
650 $aAgrobacterium
650 $aEmbryonic structures
650 $aGenetic transformation
650 $aPlant genetics
650 $aCultura de Tecido
650 $aGlycine Max
650 $aSoja
653 $aAgrobacterium-mediated transformation
653 $aEmbryonic axis
653 $aHigh-efficiency plant transformation
653 $aParticle bombardment
653 $aPlanta geneticamente modificada
653 $aRecuperação trangênica de soja
700 1 $aLOURENCO-TESSUTTI, I. T.
700 1 $aMORGANTE, C. V.
700 1 $aSANTOS, N. C.
700 1 $aPINHEIRO, L. B.
700 1 $aLINS, C. B. de J.
700 1 $aSILVA, M. C. M.
700 1 $aMACEDO, L. L. P.
700 1 $aFONTES, E. P. B.
700 1 $aGROSSI-DE-SA, M. F.
773 $tFrontiers in Plant Science$gv. 11, article 1228, 2020.
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Embrapa Recursos Genéticos e Biotecnologia (CENARGEN) |
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